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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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constrained iterative quantitative restoration method of the zeiss zen software deconvolution module  (Carl Zeiss)
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by <t>deconvolution</t> microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.
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Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

Journal: American journal of respiratory cell and molecular biology

Article Title: Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen Challenged Mice

doi: 10.1165/rcmb.2004-0060OC

Figure Lengend Snippet: Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

Article Snippet: Tissues were observed on an inverted microscope by focusing on 45 consecutive 0.2 μm focal planes followed by constrained-iterative deconvolution (DeltaVision, Applied Precision, LLC, Issaquah, Washington).

Techniques: Produced, Staining, Immunolabeling, Microscopy